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Paste Sequence :

Mark the region using '(' & ')' to slay it out and then click on "Junction format" button to generate Pre-junction & Post-junction values.
or
Specify Pre-junction & Post-junction values manually in the given text box.





Sequence Length :
Pre-Junction:
Post-Junction:
Advanced options
Probe Length: Plus or Minus
GC%:
Tm:
Tm calculation:
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