Viral proteins

Last updated: 2021 Nov 2
Total hit(s): 5
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Original Article
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The kinetics of binding of soluble ACE2 to recombinant RBD was assessed by biolayer interferometry (BLI). The affinity of the B.1.351 RBD to ACE2 was 19-fold higher when compared to the wildtype and 2.7-fold higher when compared to the alpha variant.
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 		33730597
(Cell)
PMID
33730597
Date of Publishing: 2021 Apr 29
Title Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera
Author(s) nameZhou D, Dejnirattisai W et al.
Journal Cell
Impact factor
27.35
Citation count: 406
Date of Entry 2021 Nov 2

The D614G spike mutation results in increased viral transduction. However, there was no change in the binding profiles of soluble D614 and G614 spike to human ACE2. The two dissociation constants (KD) were: KD1 = 8.45 nM and KD2 = 127 nM for D614 variant, and KD1 = 18.0 nM and KD2 = 92.7 nM for G614 variant.
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 		32587969 (33570490)
(bioRxiv)
PMID
32587969 (33570490)
Date of Publishing: 2020 Jun 15
Title The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types
Author(s) name Daniloski Z, Guo X, Sanjana NE.
Journal bioRxiv
Impact factor
N/A
Citation count: 2
Date of Entry 2021 Sep 13

Structure analysis of SARS-CoV-2 N439K Receptor Binding Domain (RBD) in complex with hACE2 at 2.8 resolution showed strong non-covalent salt bridges. These bridges enhanced the binding for hACE2 in the N439K variant, as similar to the SARS-CoV N439R variant. Double mutants of N439K/R and K417V mutations show that salt bridge loss at RBD position 417 is compensated by the one at position 439. This results in hACE2 affinity similar to the wild type. Methods used for study: X-ray structure, Surface plasmon resonance (SPR)
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 		33621484
(Cell)
PMID
33621484
Date of Publishing: 2021 Jan 28
Title Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity
Author(s) nameThomson EC, Rosen LE et al.
Journal Cell
Impact factor
27.35
Citation count: 259
Date of Entry 2021 Aug 6

The binding kinetics of the spike protein of alpha variant and spike of the D614G variant was compared by biolayer interferometry (BLI). A 2-fold decrease was observed in the dissociation constant (Kd) of spike in the alpha variant (Kd=1.3nM) compared to the D614G variant (Kd=3.1nM).
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 		Pre-print ( bioRXiv )
Title Impacts on the structure-function relationship of SARS-CoV-2 spike by B.1.1.7 mutations
Impact factor
N/A
Date of Entry 2021 Aug 6

The Y453F and L452R mutations significantly increased the binding affinity to human ACE2 (RBD parental KD = 2.05 0.26 nM; RBD Y453F KD = 0.51 0.06 nM; RBD L452R KD = 1.20 0.06 nM)
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 		Pre-print ( bioRXiv )
Title An emerging SARS-CoV-2 mutant evading cellular immunity and increasing viral infectivity
Impact factor
N/A
Date of Entry 2021 Aug 6