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RT-PCR assay
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Original Article
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Reporting a conventional one-step RT-PCR assay, specific for the
E
gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places.
The conventional PCR detected until 1e-7 dilutions of the isolates. The highest Ct detected by conventional PCR was 35.98
✍
32767275
(
Braz J Microbiol
)
PMID
32767275
Date of Publishing
: 2020 Sep
Title
Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) name
Dorlass EG, Monteiro CO et al.
Journal
Braz J Microbiol
Impact factor
1.67
Citation count
: 19
×
NLM format
Dorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, Valério CA, Chalup VN, Mello R, Almeida FJ, Aguiar AS, Barrientos ACM, Sucupira C, De Paulis M, Sáfadi MAP, Silva DGBP, Sodré JJM, Soledade MP, Matos SF, Ferreira SR, Pinez CMN, Buonafine CP, Pieroni LNF, Malta FM, Santana RAF, Souza EC, Fock RA, Pinho JRR, Ferreira LCS, Botosso VF, Durigon EL, Oliveira DBL. Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR. Braz J Microbiol. 2020 Sep;51(3):1117-1123. PMID:32767275
Reporting a conventional two-step RT-PCR assay, specific for the
E
gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places.
The conventional PCR detected until 1e-7 dilutions of the isolates. The highest Ct detected by conventional PCR was 35.98
✍
32767275
(
Braz J Microbiol
)
PMID
32767275
Date of Publishing
: 2020 Sep
Title
Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) name
Dorlass EG, Monteiro CO et al.
Journal
Braz J Microbiol
Impact factor
1.67
Citation count
: 19
×
NLM format
Dorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, Valério CA, Chalup VN, Mello R, Almeida FJ, Aguiar AS, Barrientos ACM, Sucupira C, De Paulis M, Sáfadi MAP, Silva DGBP, Sodré JJM, Soledade MP, Matos SF, Ferreira SR, Pinez CMN, Buonafine CP, Pieroni LNF, Malta FM, Santana RAF, Souza EC, Fock RA, Pinho JRR, Ferreira LCS, Botosso VF, Durigon EL, Oliveira DBL. Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR. Braz J Microbiol. 2020 Sep;51(3):1117-1123. PMID:32767275
Reporting one step nested RT-PCR, specific for
ORF1ab
gene, which performs two successive rounds of amplification at low cost with greater flexibility.
Assay efficiency was found to be 99.66%. 183 bp sequence of ORF1ab gene (region 3801 to 3983 bp) of the reference genome (accession number: MN908947.3) has 100% similarity to all SARS-CoV-2 sequences reported in the GenBank, and 86.96% similarity with the bat SARS-like coronavirus isolate sequence. The 1COFw and 1CORw primers can be used separately for COVID-19 diagnosis from clinical samples.
✍
32794453
(
J Infect Dev Ctries
)
PMID
32794453
Date of Publishing
: 2020 Jul 31
Title
One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection
Author(s) name
Meza-Robles C, Barajas-Saucedo CE et al.
Journal
J Infect Dev Ctries
Impact factor
1.26
Citation count
: 8
×
NLM format
Meza-Robles C, Barajas-Saucedo CE, Tiburcio-Jimenez D, Mokay-Ramírez KA, Melnikov V, Rodriguez-Sanchez IP, Martinez-Fierro ML, Garza-Veloz I, Zaizar-Fregoso SA, Guzman-Esquivel J, Ramirez-Flores M, Newton-Sanchez OA, Espinoza-Gómez F, Delgado-Enciso OG, Centeno-Ramirez AS, Delgado-Enciso I. One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection. J Infect Dev Ctries. 2020 Jul 31;14(7):679-684. PMID:32794453
The implementation of Nested RT-PCR for the detection of COVID-19, based on SARS-CoV-2
Orf1a
and
S
genes.
✍
32074516
(
Jpn J Infect Dis
)
PMID
32074516
Date of Publishing
: 2020 Jul 22
Title
Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) name
Shirato K, Nao N et al.
Journal
Jpn J Infect Dis
Impact factor
1.11
Citation count
: 207
×
NLM format
Shirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M. Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan. Jpn J Infect Dis. 2020 Jul 22;73(4):304-307. PMID:32074516
In this study, a clinically useful timeline of two diagnostic tests, reverse transcriptase-polymerase chain reaction and
IgM
and
IgG
enzyme-linked immunosorbent assay is reported, by using data from several published reports for detection of COVID-19. For
symptomatic
patients, viral
RNA
in the nasopharyngeal swab becomes detectable as early as day 1 of symptoms onset, peaks within the first week, declines by the third week, and subsequently becomes undetectable.
In the study, most data are for adult populations who are not immunocompromised.
✍
32374370
(
JAMA
)
PMID
32374370
Date of Publishing
: 2020 Jun 9
Title
Interpreting diagnostic tests for SARS CoV-2
Author(s) name
Sethuraman N, Jeremiah SS, Ryo A.
Journal
JAMA
Impact factor
14.78
Citation count
: 649
×
NLM format
Sethuraman N, Jeremiah SS, Ryo A. Interpreting diagnostic tests for SARS CoV-2. JAMA. 2020 Jun 9;323(22):2249-2251. PMID:32374370