Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) name
Pang B, Xu J et al.
Journal
Anal Chem
Impact factor
6.37
Citation count: 41
×
NLM format
Pang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology. Anal Chem. 2020 Dec 15;92(24):16204-16212. PMID:33238709
Inner primer pairs (FIP, BIP) for an RT-LAMP Cas12a assay specific for the N gene of SARS-CoV-2.
Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) name
Pang B, Xu J et al.
Journal
Anal Chem
Impact factor
6.37
Citation count: 41
×
NLM format
Pang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology. Anal Chem. 2020 Dec 15;92(24):16204-16212. PMID:33238709
Loop primer pairs (LF and LB) for an RT-LAMP Cas12a assay specific for the N gene of SARS-CoV-2.
Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) name
Pang B, Xu J et al.
Journal
Anal Chem
Impact factor
6.37
Citation count: 41
×
NLM format
Pang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology. Anal Chem. 2020 Dec 15;92(24):16204-16212. PMID:33238709
Outer primer pairs for an RT-LAMP Cas12a assay specific for the E gene of SARS-CoV-2.
Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) name
Pang B, Xu J et al.
Journal
Anal Chem
Impact factor
6.37
Citation count: 41
×
NLM format
Pang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology. Anal Chem. 2020 Dec 15;92(24):16204-16212. PMID:33238709
Inner primer pairs (FIP, BIP) for an RT-LAMP Cas12a assay specific for the E gene of SARS-CoV-2
Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) name
Pang B, Xu J et al.
Journal
Anal Chem
Impact factor
6.37
Citation count: 41
×
NLM format
Pang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology. Anal Chem. 2020 Dec 15;92(24):16204-16212. PMID:33238709
Loop primer pairs (LF and LB) for an RT-LAMP Cas12a assay specific for the E gene of SARS-CoV-2.
Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) name
Pang B, Xu J et al.
Journal
Anal Chem
Impact factor
6.37
Citation count: 41
×
NLM format
Pang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology. Anal Chem. 2020 Dec 15;92(24):16204-16212. PMID:33238709
Reporter labeled with fluorophore and quencher specific for the purpose of fluorescence detection carried out by Cas12a-mediated RT-LAMP assay.
Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) name
Pang B, Xu J et al.
Journal
Anal Chem
Impact factor
6.37
Citation count: 41
×
NLM format
Pang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology. Anal Chem. 2020 Dec 15;92(24):16204-16212. PMID:33238709
Sequences of the DNA probes used in the HC-FIA assay specific for binding with the N region of the SARS-CoV-2 genome.
The software Primer Premier 5.0, was used for designing the primer
Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) name
Wang D, He S et al.
Journal
Nat Biomed Eng
Impact factor
31.8
Citation count: 49
×
NLM format
Wang D, He S, Wang X, Yan Y, Liu J, Wu S, Liu S, Lei Y, Chen M, Li L, Zhang J, Zhang L, Hu X, Zheng X, Bai J, Zhang Y, Zhang Y, Song M, Tang Y. Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020 Dec;4(12):1150-1158. PMID:33273714
Sequences of the DNA probes used in the HC-FIA assay specific for binding with the ORF1ab region of the SARS-CoV-2 genome.
The software Primer Premier 5.0, was used for designing the primer
Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) name
Wang D, He S et al.
Journal
Nat Biomed Eng
Impact factor
31.8
Citation count: 49
×
NLM format
Wang D, He S, Wang X, Yan Y, Liu J, Wu S, Liu S, Lei Y, Chen M, Li L, Zhang J, Zhang L, Hu X, Zheng X, Bai J, Zhang Y, Zhang Y, Song M, Tang Y. Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020 Dec;4(12):1150-1158. PMID:33273714
The eight specific primer sequences used in RT-LAMP assay for the detection of SARS-CoV-2.
A sensitive reverse transcription loop mediated isothermal amplification assay for direct visual detection of SARS-CoV-2
Author(s) name
Lau YL, Ismail IB et al.
Journal
Am J Trop Med Hyg
Impact factor
2.25
Citation count: 4
×
NLM format
Lau YL, Ismail IB, Izati Binti Mustapa N, Lai MY, Tuan Soh TS, Hassan AH, Peariasamy KM, Lee YL, Goh PP. A sensitive reverse transcription loop mediated isothermal amplification assay for direct visual detection of SARS-CoV-2. Am J Trop Med Hyg. 2020 Dec;103(6):2350-2352. PMID:33098286
Sequences of the DNA probes used in the HC-FIA assay specific for binding with the E region of the SARS-CoV-2 genome.
The software Primer Premier 5.0, was used for designing the primer
Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) name
Wang D, He S et al.
Journal
Nat Biomed Eng
Impact factor
31.8
Citation count: 49
×
NLM format
Wang D, He S, Wang X, Yan Y, Liu J, Wu S, Liu S, Lei Y, Chen M, Li L, Zhang J, Zhang L, Hu X, Zheng X, Bai J, Zhang Y, Zhang Y, Song M, Tang Y. Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020 Dec;4(12):1150-1158. PMID:33273714
Primers sequence specific for the RdRp gene used in RT-LAMP assay to detect SARS-CoV-2.
Primers were designed by using PrimerExplorer version 5 (http://primerexplorer.jp/e/)
Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2
Author(s) name
Nawattanapaiboon K , Pasomsub E et al.
Journal
Analyst
Impact factor
3.98
Citation count: 20
×
NLM format
Nawattanapaiboon K , Pasomsub E , Prombun P , Wongbunmak A , Jenjitwanich A , Mahasupachai P , Vetcho P , Chayrach C , Manatjaroenlap N , Samphaongern C , Watthanachockchai T , Leedorkmai P , Manopwisedjaroen S , Akkarawongsapat R , Thitithanyanont A , Phanchana M , Panbangred W , Chauvatcharin S , Srikhirin T . Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2. Analyst. 2021 Jan 21;146(2):471-477. PMID:33165486
Probe for a real-time RT-PCR test specific for the nsp1 gene of SARS-CoV-2.
Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) name
Chan WM, Ip JD et al.
Journal
J Med Virol
Impact factor
2.07
Citation count: 20
×
NLM format
Chan WM, Ip JD, Chu AW, Yip CC, Lo LS, Chan KH, Ng AC, Poon RW, To WK, Tsang OT, Leung WS, Kwan MY, Chua GT, Chung TW, Hung IF, Kok KH, Cheng VC, Chan JF, Yuen KY, To KK. Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing. J Med Virol. 2020 Nov;92(11):2725-2734. PMID:32501535
Primer pairs for a real-time RT-PCR test specific for the nsp1 gene of SARS-CoV-2.
Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) name
Chan WM, Ip JD et al.
Journal
J Med Virol
Impact factor
2.07
Citation count: 20
×
NLM format
Chan WM, Ip JD, Chu AW, Yip CC, Lo LS, Chan KH, Ng AC, Poon RW, To WK, Tsang OT, Leung WS, Kwan MY, Chua GT, Chung TW, Hung IF, Kok KH, Cheng VC, Chan JF, Yuen KY, To KK. Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing. J Med Virol. 2020 Nov;92(11):2725-2734. PMID:32501535
Primer pairs, for a real-time RT-RAA assay, specific for the N gene of SARS CoV2.
A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
Author(s) name
Wu T, Ge Y et al.
Journal
Virology
Impact factor
2.819
Citation count: 14
×
NLM format
Wu T, Ge Y, Zhao K, Zhu X, Chen Y, Wu B, Zhu F, Zhu B, Cui L. A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). Virology. 2020 Oct;549:1-4. PMID:32758712
The second set of primer and probe sequences (YCH-N2) based on the Nucleocapsid gene developed by YCH (Yamanashi Central Hospital), used in real-time RT-PCR assay.
Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name
Hirotsu Y, Mochizuki H, Omata M.
Journal
J Virol Methods
Impact factor
1.76
Citation count: 29
×
NLM format
Hirotsu Y, Mochizuki H, Omata M. Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay. J Virol Methods. 2020 Oct;284:113926. PMID:32650037
The first set of primer and probe sequences (YCH-N1) based on the Nucleocapsid gene developed by YCH (Yamanashi Central Hospital), used in real-time RT-PCR assay.
The expected amplicon size is 158 bp. The assay uses double-quencher probe
A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
Author(s) name
Wu T, Ge Y et al.
Journal
Virology
Impact factor
2.819
Citation count: 14
×
NLM format
Wu T, Ge Y, Zhao K, Zhu X, Chen Y, Wu B, Zhu F, Zhu B, Cui L. A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). Virology. 2020 Oct;549:1-4. PMID:32758712
The first set of primer and probe sequences (CDC-N1) based on the Nucleocapsid gene developed by CDC, used in real-time RT-PCR assay.
The expected amplicon size is 72 bp. The assay uses single-quencher probe