| | Reporting one step nested RT-PCR, specific for ORF1ab gene, which performs two successive rounds of amplification at low cost with greater flexibility. | Assay efficiency was found to be 99.66%.
183 bp sequence of ORF1ab gene (region 3801 to 3983 bp) of the reference genome (accession number: MN908947.3) has 100% similarity to all SARS-CoV-2 sequences reported in the GenBank, and 86.96% similarity with the bat SARS-like coronavirus isolate sequence.
The 1COFw and 1CORw primers can be used separately for COVID-19 diagnosis from clinical samples. ✍ | 32794453
(J Infect Dev Ctries)
| PMID | 32794453 Date of Publishing: 2020 Jul 31 | | Title | One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection | | Author(s) name | Meza-Robles C, Barajas-Saucedo CE et al. | | Journal | J Infect Dev Ctries | | Impact factor | 1.26 Citation count: 8 |
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| NLM format |
| Meza-Robles C, Barajas-Saucedo CE, Tiburcio-Jimenez D, Mokay-Ramírez KA, Melnikov V, Rodriguez-Sanchez IP, Martinez-Fierro ML, Garza-Veloz I, Zaizar-Fregoso SA, Guzman-Esquivel J, Ramirez-Flores M, Newton-Sanchez OA, Espinoza-Gómez F, Delgado-Enciso OG, Centeno-Ramirez AS, Delgado-Enciso I. One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection. J Infect Dev Ctries. 2020 Jul 31;14(7):679-684. PMID:32794453 |
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| | The implementation of Nested RT-PCR for the detection of COVID-19, based on SARS-CoV-2 Orf1a and S genes. | ✍ | 32074516
(Jpn J Infect Dis)
| PMID | 32074516 Date of Publishing: 2020 Jul 22 | | Title | Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan | | Author(s) name | Shirato K, Nao N et al. | | Journal | Jpn J Infect Dis | | Impact factor | 1.11 Citation count: 207 |
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| NLM format |
| Shirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M. Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan. Jpn J Infect Dis. 2020 Jul 22;73(4):304-307. PMID:32074516 |
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