| | In this research study, N- and E- gene multiplex (ddCoV_N and ddCoV_E) RT-ddPCR method is redesigned and optimized to detect SARS-CoV-2 from wastewater samples and compared with the standard nCoV_N2 and E_Sarbeco RT-qPCR assays. | SARS-CoV-2 wastewater surveillance gives the community-level infection trends and screens infected individuals at the building level.
Differences within gene assays (ddCoV_N, ddCoV_E, and nCoV_N2, E_Sarbeco) were small because the primer and probe sequences were designed around the same genomic locations. ✍ | Pre-print (medRXiv)
| Title | Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR | | Author(s) name | - | | Impact factor | N/A | | Date of Entry | 2021 Jun 14 |
|
| | Reporting one-step RT-dPCR (reverse transcription digital PCR ) method based on SARS-CoV-2 ORF1ab, N, and E gene for detecting SARS-CoV-2 from pharyngeal swab samples. RT dPCR uses droplets of samples with as low as 2 gene copies to diagnose COVID-19 positive. | The Specificity of the assays were determined by testing Influenza virus and other human coronavirus and tests gave negative results. ✍ | 33379001
(Talanta)
| PMID | 33379001 Date of Publishing: 2021 Mar 1 | | Title | Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR | | Author(s) name | Dong L, Zhou J et al. | | Journal | Talanta | | Impact factor | 10.5 Citation count: 48 |
×
| NLM format |
| Dong L, Zhou J, Niu C, Wang Q, Pan Y, Sheng S, Wang X, Zhang Y, Yang J, Liu M, Zhao Y, Zhang X, Zhu T, Peng T, Xie J, Gao Y, Wang D, Dai X, Fang X. Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR. Talanta. 2021 Mar 1;224:121726. PMID:33379001 |
|
| | Reporting a newly designed droplet digital PCR (ddPCR) assay, based on RNA-dependent RNA polymerase gene (RdRp) of SARS-CoV-2. | This assay was extremely sensitive at detecting negative rtPCR samples (55 suspected SARS-CoV-2 rtPCR negative) as Positive. All positive detected clinical samples were characterized by low viral loads.
The positive SARS-CoV-2 samples detected by ddPCR were also confirmed by the Serological assay (presence of SARS-CoV-2 IgG) at the later stage of illness, results showed the presence of SARS-CoV-2 antibodies in all patients tested positive for SARS-CoV-2 in ddPCR (100%) ✍ | 32898153
(PLoS One)
| PMID | 32898153 Date of Publishing: 2020 | | Title | Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients | | Author(s) name | Alteri C, Cento V et al. | | Journal | PLoS One | | Impact factor | 2.87 Citation count: 44 |
×
| NLM format |
| Alteri C, Cento V, Antonello M, Colagrossi L, Merli M, Ughi N, Renica S, Matarazzo E, Di Ruscio F, Tartaglione L, Colombo J, Grimaldi C, Carta S, Nava A, Costabile V, Baiguera C, Campisi D, Fanti D, Vismara C, Fumagalli R, Scaglione F, Epis OM, Puoti M, Perno CF. Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients. PLoS One. 2020 Sep 8;15(9):e0236311. PMID:32898153 |
|