Diagnostics

Last updated: 2021 Jun 14
Total hit(s): 89
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In this research study, N- and E- gene multiplex (ddCoV_N and ddCoV_E) RT-ddPCR method is redesigned and optimized to detect SARS-CoV-2 from wastewater samples and compared with the standard nCoV_N2 and E_Sarbeco RT-qPCR assays. SARS-CoV-2 wastewater surveillance gives the community-level infection trends and screens infected individuals at the building level.
Differences within gene assays (ddCoV_N, ddCoV_E, and nCoV_N2, E_Sarbeco) were small because the primer and probe sequences were designed around the same genomic locations.
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 		Pre-print (medRXiv)
Title Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR
Author(s) name -
Impact factor
N/A
Date of Entry 2021 Jun 14

The research focuses on the changes in serum SARS-CoV-2 specific IgM/IgG antibody levels in asymptomatic and reoccurring positive nucleic acid test (RPNAT) group patients. The IgM levels of the RPNAT group at the time of discharge were higher than those Non-RPNAT group. SARS-CoV-2 specific IgM and IgG increased levels correlated with the positive conversion of RT-PCR retests
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 		32808808
(Ann Med)
PMID
32808808
Date of Publishing: 2021 Dec
Title Changes in serum virus specific IgM/IgG antibody in asymptomatic and discharged patients with reoccuring positive COVID-19 nucleic acid test
Author(s) nameLiu J, Lian R et al.
Journal Ann Med
Impact factor
3.01
Citation count: 11

This study reports a rapid and inexpensive diagnostic test, called RAPID 1.0 (Real-time Accurate Portable Impedimetric Detection prototype 1.0) which provides a result within 4 minutes. The RAPID technology is based on electrochemical impedance spectroscopy (EIS), which transforms the binding event between the SARS-CoV-2 spike protein and receptor protein ACE2 into a detected electrical signal. The signal can be read through a desktop instrument or a smartphone. RAPID 1.0 is a good alternative to sophisticated diagnostic technique like RT-PCR. RAPID is inexpensive compared to existing methods for SARS-CoV-2 detection
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 		33997767
(Matter)
PMID
33997767
Date of Publishing: 2021 Jul 7
Title Low-cost Biosensor for Rapid Detection of SARS-CoV-2 at the Point-of-Care
Author(s) nameTorres MDT, de Araujo WR et al.
Journal Matter
Impact factor
- n/a -
Citation count: 16

Reporting an AI-based diagnosis method that integrates Self-supervised Learning as a plugin module into a Multi-task Convolutional Neural Network for the automatic diagnosis of COVID-19 based on both CT and X-ray datasets. The three CNNs applied to diagnose COVID-19 under single task schemes are : (i) VGG-19 (ii) ResNet-50 (iii) EfficientNet
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 		33518812
(Pattern Recognit)
PMID
33518812
Date of Publishing: 2021 Jun
Title Multi-task contrastive learning for automatic CT and X-ray diagnosis of COVID-19
Author(s) nameLi J, Zhao G et al.
Journal Pattern Recognit
Impact factor
7.35
Citation count: 14

Reporting one-step RT-dPCR (reverse transcription digital PCR ) method based on SARS-CoV-2 ORF1ab, N, and E gene for detecting SARS-CoV-2 from pharyngeal swab samples. RT dPCR uses droplets of samples with as low as 2 gene copies to diagnose COVID-19 positive. The Specificity of the assays were determined by testing Influenza virus and other human coronavirus and tests gave negative results.
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 		33379001
(Talanta)
PMID
33379001
Date of Publishing: 2021 Mar 1
Title Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
Author(s) nameDong L, Zhou J et al.
Journal Talanta
Impact factor
10.5
Citation count: 48

Immunosensor based quantification of the ultralow concentration of cytokines (IL-6, TNF-alpha, etc.) present in blood or respiratory samples to monitor local and systemic inflammation. Limitation : Biosensors may have a drawback when detecting IL-6 in real matrices as proteins and mucin concentrations may interfere.
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 		33519090
(Sens Actuators B Chem)
PMID
33519090
Date of Publishing: 2021 Mar 1
Title Paper biosensors for detecting elevated IL-6 levels in blood and respiratory samples from COVID-19 patients
Author(s) nameAdrover-Jaume C, Alba-Patiño A et al.
Journal Sens Actuators B Chem
Impact factor
14.9
Citation count: 12

A new nanotechnology-based PCR-assisted colorimetric SARS-CoV-2 detection assay works on the principle of 5'-exonuclease activity of polymerase and linker-based single-component assembly of gold nanoparticle-core spherical nucleic acids. The positive and negative viral samples produce red and purple colors in the post-PCR colorimetric test, respectively.
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 		33012989
(Sens Actuators B Chem)
PMID
33012989
Date of Publishing: 2021 Feb 1
Title Conventional PCR Assisted Single-Component Assembly of Spherical Nucleic Acids for Simple Colorimetric Detection of SARS-CoV-2
Author(s) nameKarami A, Hasani M et al.
Journal Sens Actuators B Chem
Impact factor
14.9
Citation count: 15

Detection of SARS CoV-2 IgM, and IgA antibodies from Sera samples using Luminex bead-based assay by targeting SARS-CoV-2 spike and nucleocapsid antigens. The neutralizing and binding IgG, IgA, and IgM antibody levels were higher for severe than mild cases in the early convalescent phase (<6 weeks). 1/300 serum dilution showed the best signal to noise ratio for IgG and IgM and 1/150 for IgA
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 		33227340
(J Virol Methods)
PMID
33227340
Date of Publishing: 2021 Feb
Title Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay
Author(s) nameMariën J, Ceulemans A et al.
Journal J Virol Methods
Impact factor
1.76
Citation count: 41

A lateral flow assay that detects IgG and IgM antibody concentration against spike protein in two different cartridges with just maximum specificity Hepatitis and autoimmune samples were the main sources of limited cross reactions
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 		32710669
(J Med Virol)
PMID
32710669
Date of Publishing: 2021 Feb
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD et al.
Journal J Med Virol
Impact factor
2.07
Citation count: 21

A chemiluminescent assay that detects IgG antibodies against nucleocapsid gene with utmost specificity Hepatitis and autoimmune samples were the main sources of limited cross reactions
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 		32710669
(J Med Virol)
PMID
32710669
Date of Publishing: 2021 Feb
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD et al.
Journal J Med Virol
Impact factor
2.07
Citation count: 21

Compared to the SARS-CoV-2 RT-PCR assay, the SARS-CoV-2 Rapid Antigen Test (Roche, Switzerland) showed less sensitivity and specificity. The Rapid Antigen test provided an advantage of quick detection of infected people.
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 		33227341
(J Virol Methods)
PMID
33227341
Date of Publishing: 2021 Feb
Title Comparision of the SARS-CoV-2 rapid antigen test to real star SARS-CoV-2 RT-PCR kit
Author(s) nameKrüttgen A, Cornelissen CG et al.
Journal J Virol Methods
Impact factor
1.76
Citation count: 60

A compariosn of three serological tests that detect antibody levels of IgG, Ig and IgA show high assasy specificity and sensitivity without any indication of cross-reaction. Hepatitis and autoimmune samples were the main sources of limited cross reactions
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 		32710669
(J Med Virol)
PMID
32710669
Date of Publishing: 2021 Feb
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD et al.
Journal J Med Virol
Impact factor
2.07
Citation count: 21

In this study, the Rapid Antigen detection kit, Roche SARS-CoV-2 Rapid Antigen Test (RAT), a chromatographic immunoassay, was evaluated for the rapid detection of SARS-CoV-2 N protein in PCR-positive and PCR-negative swab samples. The kit produced false-negative results, with overall clinical sensitivity and specificity of 50.3% and 97.7%, respectively. RAT test was extensively flexible with the way samples were stored and used for testing.
The use of oropharyngeal sample as an alternative is not beneficial for RAT test.
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 		33452927
(Med Microbiol Immunol)
PMID
33452927
Date of Publishing: 2021 Jan 16
Title Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting
Author(s) nameOsterman A, Baldauf HM et al.
Journal Med Microbiol Immunol
Impact factor
6.7
Citation count: 30

In this study, the Rapid Antigen detection kit, STANDARD F COVID-19 Ag FIA (FIA), a fluorescent immunoassay, was evaluated for the rapid detection of SARS-CoV-2 nucleoprotein in PCR-positive and PCR-negative swab samples. The kit produced false-negative results, with overall clinical sensitivity and specificity of 45.4% and 97.8%, respectively. Prior storage of the specimen at -20°C slightly enhances the rate of SARS-CoV-2 antigen test.
Also the oropharyngeal can be used as an alternative sampling site for FIA.
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 		33452927
(Med Microbiol Immunol)
PMID
33452927
Date of Publishing: 2021 Jan 16
Title Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting
Author(s) nameOsterman A, Baldauf HM et al.
Journal Med Microbiol Immunol
Impact factor
6.7
Citation count: 30

In this study, the diagnostic performance of the structured approach of chest radiograph was evaluated and compared with RT-PCR. The study revealed that positive chest radiography had a high likelihood of predicting a positive RT-PCR. The accuracy of baseline CXR in the diagnosis of COVID-19 is moderate to good, with high specificity and low sensitivity. The Chest radiographs (CXR) are an alternative to the CT scans taken for the COVID-19 diagnosis.
The graphs obtained have low sensitivity hence further validation is required.
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 		33246271
(Eur J Radiol)
PMID
33246271
Date of Publishing: 2021 Jan
Title Structural reporting in portable chest radiographs : An essential tool in the diagnosis of COVID-19
Author(s) nameYates A, Dempsey PJ et al.
Journal Eur J Radiol
Impact factor
3.11
Citation count: 6

A RT loop mediated isothermal amplification technology combined with CRISPR-Cas12 based detection of SARS-Cov-2 showed improved specificity against N and E genes and assay sensitivity. Additional information: (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724759/bin/ac0c04047_si_001.pdf)
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 		33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 41

Reporting an amplification-free, fluorescence-based diagnostic method, namely Hybrid Capture Fluorescence Immunoassay (HC-FIA), for diagnosing SARS-CoV-2. It is carried on a lateral flow strip and targets ORF1ab, E, and the N regions of the genome to detect SARS-CoV-2. The assay resulted in 100% and 99% sensitivities and specificities, respectively. This assay is based on the capture of RNA-DNA hybrids and on immunofluorescence analysis. The SARS-CoV-2 HC-FIA detection system Fig.1 (https://www.nature.com/articles/s41551-020-00655-z#Fig1)
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 		33273714
(Nat Biomed Eng)
PMID
33273714
Date of Publishing: 2020 Dec
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S et al.
Journal Nat Biomed Eng
Impact factor
31.8
Citation count: 49

Reporting a new, fast detection method, NanoPCR assay specific for the N1 and N2 gene of SARS-CoV-2 based on plasmonic RT-PCR with in-situ fluorescence signal detection. Pearson coefficient (r) values indicates strong positive correlation between two variables.
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 		33273713
(Nat Biomed Eng)
PMID
33273713
Date of Publishing: 2020 Dec
Title Fast detection of SARS CoV-2 RNA via the integration of plasmonic thermocycling and fluoroscence detection in a portable device
Author(s) nameCheong J, Yu H et al.
Journal Nat Biomed Eng
Impact factor
31.8
Citation count: 36

Reporting a highly sensitive and specific one-pot fluorescence-based detection assay, SENSR assay (sensitive splint-based one-pot isothermal RNA ) based on RdRp gene to detect SARS-CoV-2. Clinical samples were pre-treated by either thermal (heated to 95degree Celsius for 5min) or chemical lysis (with Nonidet P-40 for 5 min) and mixed directly with the SENSR mixture.
✍
 		32948855
(Nat Biomed Eng)
PMID
32948855
Date of Publishing: 2020 Dec
Title Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription
Author(s) nameWoo CH, Jang S et al.
Journal Nat Biomed Eng
Impact factor
31.8
Citation count: 43

Reporting a visual detection of SARS-CoV-2 by a rapid, simple, sensitive, and specific RT-LAMP method with the eight primer sets. One major limitation of this assay is the designing of primers for effecient LAMP RT-PCR results.
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 		33098286
(Am J Trop Med Hyg)
PMID
33098286
Date of Publishing: 2020 Dec
Title A sensitive reverse transcription loop mediated isothermal amplification assay for direct visual detection of SARS-CoV-2
Author(s) nameLau YL, Ismail IB et al.
Journal Am J Trop Med Hyg
Impact factor
2.25
Citation count: 4

Reporting SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) method to detect SARS-CoV-2, which relies on the RT-RPA method to amplify viral gene segments, followed by CRISPR-Cas13a-mediated detection of amplified genes. The assay was 100% specific, and 96% sensitive with the fluorescence readout, and 88% sensitive with the lateral-flow readout. 1. SHERLOCK-based detection of the SARS-CoV-2 S gene is specific to SARS-CoV-2, with no cross-reactivity towards other common human coronaviruses 2. Table S3. RPA primers, crRNAs, and RNA reporters information(https://static-content.springer.com/esm/art%3A10.1038%2Fs41551-020-00603-x/MediaObjects/41551_2020_603_MOESM1_ESM.pdf)
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 		32848209
(Nat Biomed Eng)
PMID
32848209
Date of Publishing: 2020 Dec
Title Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
Author(s) namePatchsung M, Jantarug K et al.
Journal Nat Biomed Eng
Impact factor
31.8
Citation count: 138

In this study, a colorimetric RT-LAMP method targeting the RdRp gene is developed for detecting SARS-CoV-2 within 60 min. The results showed high sensitivity and specificity of 95.74% and 99.95%, respectively. The assay result is comparable to that of commercial qRT-PCR assays implying its beneficial use as a diagnostic method for COVID-19 screening.
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 		33165486
(Analyst)
PMID
33165486
Date of Publishing: 2020 Nov 9
Title Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2
Author(s) nameNawattanapaiboon K , Pasomsub E et al.
Journal Analyst
Impact factor
3.98
Citation count: 20

Reporting an optimized protocol serum-based enzyme-linked immunosorbent assay (ELISA) to detect antibodies against SARS-CoV-2 in saliva samples. The assay gave a sensitivity of 84.2% and a specificity of 100% in a general symptomatic population. Among two test kits tested, Gold Standard Diagnostics (GSD) and EuroImmun (EI), EI IgG and IgA kits were 90 and 86 percent sensitive, respectively, and GSD IgG and IgA kits were 69 and 15 percent sensitive, respectively, based on cutoff values for serum supplied by the manufacturers. So, the EI IgG kit was selected for saliva sample optimization experiments.
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 		33257702
(Sci Rep)
PMID
33257702
Date of Publishing: 2020 Nov 30
Title ELISA detection of SARS-CoV-2 antibodies in saliva
Author(s) nameMacMullan MA, Ibrayeva A et al.
Journal Sci Rep
Impact factor
4.12
Citation count: 29

An alternative method for the detection of SARS-CoV-2 directly from clinical specimens by application of direct Oxford Nanopore long-read third-generation metatranscriptomic and metagenomic sequencing technology. mNGS provided valuable information about the patients' respiratory microbiome and its relationship to coinfections. No SARS-CoV-2 reads were aligned to any of the ten samples that were suspected of having SARS-CoV-2 infection but were negative by RT-PCR
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 		33219095
(mBio)
PMID
33219095
Date of Publishing: 2020 Nov 20
Title Metagenomic Next-Generation Sequencing of Nasopharyngeal Specimens Collected from Confirmed and Suspect COVID-19 Patients
Author(s) nameMostafa HH, Fissel JA et al.
Journal mBio
Impact factor
6.5
Citation count: 39

Evaluating the performance of CE-labeled variplexTM real-time SARS-CoV-2 RT-LAMP assay using extracted RNA in comparison with commercial Corman's LightMix E gene RT-PCR as reference. Variplex RT-LAMP assay showed a lower sensitivity, compared to commercial E gene RT-PCR, but sensitivity increased (86.4%) when samples with E gene Ct values <35 were considered.
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 		32891938
(J Clin Virol)
PMID
32891938
Date of Publishing: 2020 Nov
Title Use of the variplex SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis
Author(s) nameRödel J, Egerer R et al.
Journal J Clin Virol
Impact factor
2.95
Citation count: 33